セラミックハイパーD Fイオン交換クロマトグラフィー担体(Q, DEAE, CM) product photo Primary L

セラミックハイパーD Fイオン交換クロマトグラフィー担体(Q, DEAE, CM)

Preparative Sorbents for the Purification
of Biomolecules by Charge

  • High binding capacity. “Gel-in-a-shell” design delivers outstanding dynamic capacity and exceptional dimensional stability for unsurpassed productivity.
  • High flow rates. Proteins diffuse rapidly within the hydrogel, facilitating rapid uptake of product. This mechanism of mass transfer (known as “enhanced diffusion”) allows the sorbents to operate free of constraints.
  • Abundant ion exchange sites in the hydrogel are highly accessible to protein molecules.
  • Does not shrink or swell in response to changes in pH, ionic strength, or flow rate.
  • Rigid, non-compressible sorbents are easy to pack.
  • Easy cleaning with sodium hydroxide.
  • Scalable from research and development to manufacturing.



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概要

Preparative Sorbents for the Purification
of Biomolecules by Charge

  • High binding capacity. “Gel-in-a-shell” design delivers outstanding dynamic capacity and exceptional dimensional stability for unsurpassed productivity.
  • High flow rates. Proteins diffuse rapidly within the hydrogel, facilitating rapid uptake of product. This mechanism of mass transfer (known as “enhanced diffusion”) allows the sorbents to operate free of constraints.
  • Abundant ion exchange sites in the hydrogel are highly accessible to protein molecules.
  • Does not shrink or swell in response to changes in pH, ionic strength, or flow rate.
  • Rigid, non-compressible sorbents are easy to pack.
  • Easy cleaning with sodium hydroxide.
  • Scalable from research and development to manufacturing.

用途
  • Direct capture of biomolecules from a variety of feedstocks
  • Purification of polypeptides, IgG, and albumin
  • Large-scale purifications
  • Purification of monoclonal antibodies from ascites or cell culture
  • Plasmid purification
  • Process polishing steps
仕様
 
Type of Ceramic HyperD F Resin Q DEAE CM
Grade F F F
Particle Size (µm) ~50 ~50 ~50
Dynamic Binding Capacity (mg/mL)
10% Breakthrough at 200 cm/h
BSA 851 BSA 851 IgG 602
Amount of Ionic Groups (µeq/mL) 250 200 250 - 400
Working pH 2 - 12 2 - 12 2 - 12
Cleaning pH 1 - 14 1 - 14 1 - 14
Volume Changes Due to
pH and Ionic Strength

Non -compressible

Pressure Resistance 20 grade: 200 bar (20,000 kPa, 2,901 psi) F grade: > 70 bar (7,000 kPa, 1,015 psi)
Storage Temperature

2 - 30 °C (36 - 56 °F)
2 - 8 °C (36 - 46°F) after opening

 

1Sample: 5 mg/mL BSA in 50 mM Tris-HCl buffer, pH 8.6
2Sample: 5 mg/mL Human IgG in 50 mM sodium acetate, 100 mM NaCl, pH 4.7
性能

Dynamic Binding Capacity of Ceramic HyperD F Ion Exchange Resin Packed in 1 mL Chromatography Glass Columns at a Range of Flow Rates (Linear Velocities)

 
  Dynamic Binding Capacity (mg/mL)1
Media 1 mL/min (258 cm/h) 5 mL/min (1290 cm/h) 10 mL/min
HyperD F DEAE 101.5 87.5 77.5
HyperD F CM  108.0 87.5 73.5
 
1Dynamic binding capacity measured by breakthrough curve analysis at 10% of media saturation; a 1 mL volume column of ion exchange resin was packed and equilibrated with 25 mM Tris HCl pH 8.5 (anion ion exchange) or 10 mM MES-NaOH pH 5.5 (cation ion exchange) at the flow rates of 1, 5, or 10 mL/min. For anion ion exchange, 5 mg/mL BSA in the above buffer was then pumped onto the column until a break through in absorbance at 280 nm was seen. The flow was continued until a plateau in absorbance was achieved corresponding to 100% protein feed. Dynamic binding capacity was then calculated at 10% of the plateau value, correcting for any "dead volume" in the system and expressed as mg BSA/mL media volume. For cation ion exchange, 5 mg/mL lysozyme was used to test these resins in a similar manner to the anion ion exchange media. 
製品タイプ
Chromatography Sorbent
製品使用分野
Ion Exchange Chromatography
産業分野
Protein Sample Prep & Detection