ナノセップ & ナノセップ MF 遠心ろ過デバイス

Simple, Reliable Concentrating and
Desalting of 50 to 500 µL Samples

  • Ensures rapid processing of samples 
  • Typical recoveries are > 90%
  • Available with low proteinbinding Omega™, Bio-Inert®, and GHP membranes
  • A wide range of MWCOs, color-coded for easy identification
  • Constructed of low-binding polypropylene 
  • Ultrasonically welded seals prevent bypass or seal failure
  • Fits standard centrifuge rotors that accept 1.5 mL tubes


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概要

Simple, Reliable Concentrating and
Desalting of 50 to 500 µL Samples

  • Ensures rapid processing of samples 
  • Typical recoveries are > 90%
  • Available with low proteinbinding Omega™, Bio-Inert®, and GHP membranes
  • A wide range of MWCOs, color-coded for easy identification
  • Constructed of low-binding polypropylene 
  • Ultrasonically welded seals prevent bypass or seal failure
  • Fits standard centrifuge rotors that accept 1.5 mL tubes
用途
  • Concentrate, purify, and desalt oligonucleotides, DNA, RNA, and proteins
  • Clean up labeling and PCR reactions
  • Isolate DNA from agarose gel slices
  • Separate oligonucleotides and RNA from acrylamide gels
  • Concentrate PCR products regardless of size with 30K device if primer removal is required
仕様

Materials of Construction

  • Nanosep Devices
       Filter Media: Omega (modified polyethersulfone) ultrafiltration membrane
       Sample Reservoir, Membrane Support Base, and Filtrate Receiver: Polypropylene
  • Nanosep MF Devices
       Filter Media: Bio-Inert (modified nylon) and GHP (hydrophilic polypropylene) membranes
       Sample Reservoir, Membrane Support Base, and Filtrate Receiver: Polypropylene

Effective Filtration Area

  • 0.3 cm2

Dimensions

  • Overall Length (Fully Assembled with Cap): 4.5 cm (1.8 in.)

Capacities

  • Maximum Sample Volume: 500 µL
  • Final Concentrate Volume: 15 µL
  • Filtrate Receiver Volume: 500 µL
  • Hold-up Volume (Membrane/Support): < 5 µL

Operating Temperature Range

  • 0 - 40 °C (32 - 104 °F)

pH Range

  • Nanosep Devices: 1 - 14
  • Nanosep MF Devices: 3 - 14

Maximum Centrifugal Force

  • 14,000 x g

Centrifuge

  • Fits rotors that accept 1.5 mL tubes

Sanitization

  • Provided non-sterile. May be sanitized by filtering 70% ethanol through the device prior to use.
性能

DNA Recovery as a Function of Device MWCO


Nanosep Centrifugal Device: DNA Recovery as a Function of Device MWCO
 
A 500 μL sample of a 100 μg/mL DNA fragment solution containing 50 and 500 bp double-stranded DNA fragments was centrifuged at 5,000 x g in Nanosep devices to a final volume of 50 μL. Recovered samples were quantitated using absorbance at 260 nm. The 100K device was able to differentiate between the sizes of the DNA fragments.

Omega™ Membrane Has the Lowest Non-Specific DNA Binding


Omega™ Membrane Has the Lowest Non-Specific DNA Binding

A 500 μL sample of a 32P-labeled PCR product (400 bp, 50 ng/mL) was centrifuged at 5,000 x g in a 100K Nanosep or competitive device. The retained DNA was recovered in 40 μL TE (10 mM Tris, 1 mM EDTA, pH 8). The remaining radioactivity in the device was counted in a scintillation counter. A value at 1000 CPM roughly corresponds to 1% of the total radioactive sample. Omega membrane has the lowest non-specific binding, resulting in highest DNA recoveries. In general, DNA molecular weights do not correspond well with the MWCO because DNA is a long linear molecule. Effective retention of DNA by an ultrafiltration membrane requires a reduction in g-force to 5,000 x g. Otherwise, DNA can be forced through many MWCO membranes regardless of size.

Centrifugal Device Spin Times


Nanosep Centrifugal Device Spin Times
製品タイプ
Centrifugal Device
製品使用分野
Ultrafiltration, Microfiltration
産業分野
Analytical QC
アプリケーション
HPLC/UHPLC Sample Preparation